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Overview
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This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants.
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- Properties
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Overview
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug DARS antibody (STJ28657). Western blot was performed from the immunoprecipitate using DARS antibody (STJ28657) at a dilition of 1:1000.
Immunohistochemistry of paraffin-embedded mouse brain using DARS antibody (STJ28657) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DARS antibody (STJ28657) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using DARS antibody (STJ28657) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using DARS Antibody (STJ28657) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Anti-DARS antibody
Anti-DARS antibody
Catalog Number:
A001036737STJ
Mfr. No.:
STJ28657
Price:
$297
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