Name: Anti-CSX1/NKX2-5 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; cow; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Homeobox Protein Nkx-2.5; Cardiac-Specific Homeobox; Homeobox Protein Csx; Homeobox Protein Nk-2 Homolog E (Gene ID:1482; UniProt:P52952)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Approx 40-45kDa band observed in Mouse Heart lysates. Preliminary experimants also showed an apprix 45kDa band in Human and Rat Heart kysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies :920-9.; PMID: 8888684). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature.
    IF-Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.