STJ71309 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear st STJ71309 Immunofluorescence analysis of paraformaldehyde fixed Neuro2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane, and vesicle staining. The nucl STJ71309 (1µg/ml) staining of Human Colon (A), Cerebellum (B), Rat Brain (C) and Mouse spinal Cord (D) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CRHR1/CRF-R antibody

Catalog Number:

A001043095STJ

Mfr. No.:

STJ71309

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

Add to Cart

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Corticotropin-Releasing Factor Receptor 1; Crf-R-1; Crf-R1; Crfr-1; Corticotropin-Releasing Hormone Receptor 1; Crh-R-1; Crh-R1 (Gene ID:104909134; UniProt:P34998)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Approx 60kDa band observed in Human Brain, Colon, Rat Brain and Mouse spinal cord lysates. The observed molecular weight corresponds to earlier findings in literature with different antibodies :485-92; PMID: 11179443). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
    IF-Expression of the protein seen in the membranes, vesicles and cytoplasm of MCF7 and Neuro2a cells. Recommended concentration: 10µg/ml.

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