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Overview
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Collagen IV is a major constituent of the basement membranes along with laminins. It is composed of α1(IV) chain and α2(IV) chain in 2:1 ratio. It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas. PHM‐12 is highly specific to type IV collagen. In kidney, It reacts with glomerular and tubular basement membranes, parts of mesangial matrix and the Bowman’s capsule. It also reacts with basal lamina of capillaries as well as basement membranes in a variety of tissues. PHM‐12 is excellent for the staining of routine formalin‐fixed, paraffin embedded tissues.
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