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Overview
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Collagen III is a protein encoded by the COL3A1 gene which is approximately 138, 5 kDa. Collagen III is secreted into the extracellular matrix. It is involved in collagen chain trimerization, the integrin pathway, ERK signalling and the phospholipase-C pathway. It occurs in most soft connective tissues along with type I collagen. It is involved in regulation of cortical development and is the major ligand of ADGRG1 in the developing brain, binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13. Collagen III is expressed in the skin, pancreas, bone, liver and lung. Mutations in the COL3A1 gene may result in Ehlers-Danlos syndrome. STJ97025 was developed from clone Q76 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous Collagen III protein.
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Overview
Western blot analysis of lysates from 1) MCF7, 2) Hela, 3) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
Western blot analysis of lysates from 1) MCF7, 2) Hela, 3) 293T cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
Western blot analysis of Hela, diluted at 1:1000.
Immunofluorescence analysis of Mouse-liver tissue. 1, Collagen III monoclonal antibody (Q76) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, Collagen III monoclonal antibody (Q76) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Collagen III monoclonal antibody (Q76) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Collagen III monoclonal antibody (Q76) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Anti-Collagen III antibody
Anti-Collagen III antibody
Catalog Number:
A001052394STJ
Mfr. No.:
STJ97025
Price:
$224
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