STJ71327 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. Acti STJ71327 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Anti-CLEC16A antibody

Catalog Number:

A001043113STJ

Mfr. No.:

STJ71327

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

Add to Cart

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Protein Clec16a; C-Type Lectin Domain Family 16 Member A (Gene ID:23274; UniProt:Q2KHT3)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:16000.
    WB-Preliminary experiments gave an approx 70kDa band in Rat Testis lysates after 0.5µg/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we currently cannot find an explanation in the literature for this band, given the calculated size of 115kDa according to Rat XP_213209.4.
    IF-Strong expression of the protein seen in the nuclei and weak expression seen in the cytoplasm of A549 cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

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