Name: Anti-CD274/PD-L1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Programmed Cell Death 1 Ligand 1; Pd-L1; Pdcd1 Ligand 1; Programmed Death Ligand 1; Hpd-L1; B7 Homolog 1; B7-H1; Cd Antigen Cd274 (Gene ID:29126; UniProt:Q9NZQ7)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Approx 40kDa band observed in Human Heart lysates. The observed molecular weight corresponds to glycosylation. An additional band was also consistently observed at 55kDa, and was successfully blocked by incubation with the immunizing peptide. Preliminary testing also showed the 37+55kDa bands in lysates of cell lines, A549, Daudi, HeLa, HepG2 and Jurkat Recommended concentration: 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.
    IHC-In paraffin embedded Human Placenta shows membranous staining of cytotrophoblasts. Recommended concentration: 2-4µg/ml.
    IF-Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.