Name: Anti-Cytochrome b reductase 1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; dog; pig; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Cytochrome B Reductase 1; Duodenal Cytochrome B; Ferric-Chelate Reductase 3 (Gene ID:79901; UniProt:Q53TN4)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:16000.
    WB-Preliminary experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1µg/ml antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfully used in WB on Human in PMID: 24894955, 21973163 and 18830567.
    IHC-In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5µg/ml. Paraffin embedded Human Small Intestine. Recommended concentration: 2.5ug/ml.

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