Name: Anti-Caveolin 1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; pig
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Caveolin-1 (Gene ID:857; UniProt:Q03135)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:8000.
    WB-Approx 22kDa band observed in lysates of cell lines A431 and A549 and in Human Heart lysates. Recommended concentration: 0.001-0.01µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig Heart and Spleen, and Mouse and Rat Lung for this particular batch.
    IF-Strong expression of the protein seen in the Golgi and membranes of A431 cells and in the cytoplasm and Golgi of U2OS cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

STJ70728 (0.003µg/ml) staining of A431 (A) and (0.001ug/ml) A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

STJ70728 (0.003µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Caveolin 1 antibody

Anti-Caveolin 1 antibody

STJ70728 (0.003µg/ml) staining of A431 (A) and (0.001ug/ml) A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

STJ70728 (0.003µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Caveolin 1 antibody

Anti-Caveolin 1 antibody

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