Name: Anti-CA IX antibody
Price: 224.00 USD

Overview
- CA IX is a protein encoded by the CA9 gene which is approximately 49, 6 kDa. CA IX is localised to the nucleus and cytoplasm. It is involved in CDK-mediated phosphorylation and removal of Cdc6, nitrogen metabolism, angiogenesis and glucose metabolism. It is a zinc metalloenzyme that catalyses the reversible hydration of carbon dioxide. It participates in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of cerebrospinal fluid, saliva, and gastric acid. CA IX is expressed in the intestine, stomach, gall bladder and kidney. Mutations in the CA9 gene may result in kidney cancer and hypoxia. STJ96978 was developed from clone 12F10 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous CA IX proteins.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG1
  
  Reactivity
  
  human
  
  Clone
  
  12F10
  
  Immunogen
  
  Synthetic Peptide
  
  Purification
  
  The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  PBS, pH 7.4, containing 0.5% BSA, 0.02% sodium azide and 50% Glycerol.
  
  Storage
  
  Store at -20°C and avoid repeat freeze-thaw cycles.
  
  Regulatory Status
  
  RUO
  
  Antigen
  
  Carbonic Anhydrase 9; Carbonate Dehydratase Ix; Carbonic Anhydrase Ix; Ca-Ix; Caix; Membrane Antigen Mn; P54/58n; Renal Cell Carcinoma-Associated Antigen G250; Rcc-Associated Antigen G250; Pmw1 (Gene ID:768; UniProt:Q16790)
  
  * For research use only

Applications

Application

IF; WB; IHC (paraffin); IP

Application	Dilutions
IF	1:50-200
WB	1:3000
IP	1:200
IHC	1:50-300

We Also Recommend

Anti-Caspase 9 antibody

$224

Anti-ERCC1 antibody

$295

Anti-ERCC1 antibody

$297

Anti-ERCC1 antibody

$499

Anti-ERCC1 antibody

$499

Anti-ERCC1 antibody

$224

Anti-ERCC1 antibody

$224

Anti-ERCC1 antibody

$224

Anti-HER2 antibody

$224

Anti-HER2 antibody

$224

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

1) Input: Hela Cell Lysate , 2) IP product: IP dilute 1:200 Western blot analysis of 1) Hela, 2) 293T, diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-CA IX antibody

Anti-CA IX antibody