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STJ70684 (0.1µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

STJ70684 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b

Anti-BID antibody

Anti-BID antibody

Catalog Number:

A001042489STJ

Mfr. No.:

STJ70684

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Bh3-Interacting Domain Death Agonist; P22 Bid; Bid Cleaved Into-Bh3-Interacting Domain Death Agonist P15; P15 Bid-Bh3-Interacting Domain Death Agonist P13; P13 Bid-Bh3-Interacting Domain Death Agonist P11; P11 Bid (Gene ID:637; UniProt:P55957)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:8000.
    WB-Approx 26+24kDa bands observed in A431 cell lysates. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
    IF-Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10µg/ml.

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