Name: Anti-B7-H4 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    V-Set Domain-Containing T-Cell Activation Inhibitor 1; B7 Homolog 4; B7-H4; B7h.5; Immune Costimulatory Protein B7-H4; Protein B7s1; T-Cell Costimulatory Molecule B7x (Gene ID:79679; UniProt:Q7Z7D3)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:64000.
    WB-Approx 26+21kDa bands observed in HEK293 cell lysates, which were both successfully blocked by incubation with the immunizing peptide. An approx 26kDa and 21kDa band observed in Human Adrenal Gland lysates, and approx 27kDa in Human Pancreas lysates. Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
    IF-Strong expression of the protein seen in the cytoplasm and membranes/cell junctions of MCF7 cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.