Name: Anti-BHMT antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Betaine--Homocysteine S-Methyltransferase 1 (Gene ID:635; UniProt:Q93088)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:16000.
    WB-Approx 45kDa band observed in Rat Liver lysates. This product has been successfully used in WB on Human. This product has been successfully used in WB on Mouse. This product has been successfully used in WB on Rat 65-73 and PMID:30746538). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
    IHC-Paraffin embedded Human Liver and Kidney. Recommended concentration: 5µg/ml.