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Overview
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Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
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- Properties
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Overview
Confocal immunofluorescence analysis of U2OS cells using ATP5H Polyclonal Antibody (STJ26670) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse kidney using ATP5H Rabbit pAb (STJ26670) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using ATP5H Rabbit pAb (STJ26670) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ATP5H antibody (STJ26670) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
Anti-ATP5H antibody
Anti-ATP5H antibody
Catalog Number:
A001035527STJ
Mfr. No.:
STJ26670
Price:
$297
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