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Overview
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This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.
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Overview
Immunofluorescence analysis of HeLa cells using ATF4 antibody (STJ22704) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of HeLa cells, using ATF4 antibody (STJ22704) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 3min.
Anti-ATF4 antibody
Anti-ATF4 antibody
Catalog Number:
A001033366STJ
Mfr. No.:
STJ22704
Price:
$297
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