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Overview
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ASC is a protein encoded by the PYCARD gene which is approximately 21, 6 kDa. ASC is localised to the cytoplasm, endoplasmic reticulum, mitochondrion and nucleus. It is involved in toll-like receptor signalling pathways, CLEC7A (Dectin-1) signalling and the innate immune system. It functions as key mediator in apoptosis and inflammation. It promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a cell type-specific manner. ASC is widely expressed at low levels in human tissues. Mutations in the PYCARD gene may result in chronic recurrent multifocal osteomyelitis and Cinca syndrome. STJ91730 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of ASC protein.
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Overview
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using ASC Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from RAW264.7 cells, using ASC Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of various cells using ASC Polyclonal Antibody diluted at 1：500 Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, ASC Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, ASC Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute Plasma Membrane Protein Isolation and Cell Fractionation Kit (SM-005, Inventbiotech, MN, USA).
Western blot analysis of lysates from 1) K562, 2) Hela, 3) 3T3 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
Anti-ASC antibody
Anti-ASC antibody
Catalog Number:
A001047101STJ
Mfr. No.:
STJ91730
Price:
$224
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