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Overview
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ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
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Overview
Immunofluorescence analysis of HeLa cells using ARF1 antibody (STJ22666) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded human prostate using ARF1 antibody (STJ22666) (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ARF1 antibody (STJ22666) (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using ARF1 antibody (STJ22666) (40x lens).
Western blot analysis of extracts of various cell lines, using ARF1 antibody (STJ22666) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.
Anti-ARF1 antibody
Anti-ARF1 antibody
Catalog Number:
A001033345STJ
Mfr. No.:
STJ22666
Price:
$297
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