Name: Anti-ANGPT1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; cow; pig
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Angiopoietin-1; Ang-1 (Gene ID:284; UniProt:Q15389)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; WB; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-In transfected HEK293 transiently expressing full-length Human ANGPT1, a band of approx. 48kDa was observed. No bands were observed in mock-transfected HEK293 and the 48kDa plus a 75kDa band was observed using anti-DYKDDDDK tag antibody. An approx. 48kDa band was also observed in lysates of cell line K562 and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
    IF-Strong expression of the protein seen in the plasma membranes of A431 and HeLa cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.