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anti-AAV9 (intact particle) mouse monoclonal, ADK9, supernatant

anti-AAV9 (intact particle) mouse monoclonal, ADK9, supernatant

Catalog Number:

A001003140PRO

Mfr. No.:

651162

Price:

$506

Size:

5 mL

Quantity:

Add to Cart:

Overview
- Useful for analysis of the AAV9 assembly process. ADK9 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Not for use in WB.
  The ADK9 antibody is used as capture and detection antibody in the AAV9 Titration ELISA (PRAAV9) and the AAV9 Xpress ELISA (PRAAV9XP).
  
  Please contact us at for specific academic pricing.
  
  Background
  
  For characterization of different stages of infection and very useful for the analysis of the AAV9 assembly process. ADK9 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. The antibody cannot be used for immunoblotting.
  
  More Details
  
  AAV Antibodies
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgA kappa
  
  Reactivity
  
  virus (AAV9)
  
  Clone
  
  ADK9
  
  Immunogen
  
  AAV9 capsids
  
  Purification
  
  hybridoma cell culture supernatant
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  contains 0.09% sodium azide
  
  Storage
  
  short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
  
  * Research use only

Applications

Application

Dot Blots; ELISA; ICC/IF; Neutralization Assay

Application	Dilutions
Dot Blot	1:20 - 1:100 (non-denaturing conditions)
ELISA	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Neutralization Assay	assay dependent

Application	Species	Publication
Dot Blots	AAV9	PMID: 27424005
ELISA	AAV9	PMID: 24299301
ICC/IF	AAV9	PMID: 21956692
Neutralization Assay	AAV9	PMID: 21917944

Reference
- Tseng, Y.-S. et al. Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies. J. Virol. Methods 236, 105-110 (2016).
  Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).
  Varadi, K. et al. Novel random peptide libraries displayed on AAV serotype 9 for selection of endothelial cell-directed gene transfer vectors. Gene Ther. 19, 800-809 (2012).
  Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686-12697 (2011).

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