anti-AAV5 (intact particle) mouse monoclonal, ADK5a, lyophilized, purified

Name: anti-AAV5 (intact particle) mouse monoclonal, ADK5a, lyophilized, purified
Price: 704.00 USD

Catalog Number:

A001003125PRO

Mfr. No.:

610148

Price:

$704

Size:

50 µg

Quantity:

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Overview
- Specifically detects intact AAV5 particles, both empty and full capsids. ADK5a recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes. The antibody is not suitable for Immunoblotting.
  The ADK5a antibody is used as capture and detection antibody in the AAV5 Titration ELISA (PRAAV5).
  
  Please contact us at for specific academic pricing.
  
  Background
  
  For characterization of different stages of infection and very useful for the analysis of the AAV5 assembly process. ADK5a specifically reacts with intact adeno-associated virus 5 particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
  The antibody is usefull for neutralizing experiments.
  
  More Details
  
  AAV Antibodies
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG2a kappa
  
  Reactivity
  
  virus (AAV5)
  
  Clone
  
  ADK5a
  
  Immunogen
  
  AAV5 capsids
  
  Purification
  
  protein A affinity chromatography
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
  
  Storage
  
  short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
  
  * Research use only

Applications

Application

Affinity Chromatography; ELISA; ICC/IF; IP

Application	Dilutions
Affinity Chromatography	assay dependent
Dot Blot	assay dependent (non-denaturing conditions)
ELISA	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	1:20
Immunoprecipitation (IP)	1:5
Neutralization Assay	assay dependent

Application	Species	Publication
neutralization	AAV5	Article URL
epitope mapping	AAV5	Article URL
dot blot	AAV5	Article URL
ELISA dot blot IP	AAV5	Article URL

Reference
- Falese, L. et al. Strategy to detect pre-existing immunity to AAV gene therapy. Gene.Ther. 24, 768-778 (2017)
  Tseng, Y.-S. et al. Adeno-Associated Virus Serotype 1 (AAV1)-and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity. J. Virol. 89, 1794-1808 (2015).
  Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).
  Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17-24 (2007).