anti-AAV1 (intact particle) mouse monoclonal, ADK1a, lyophilized, purified

Overview
- Specifically detects AAV1 intact virus particles, both empty and full capsids. ADK1a recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. Not suitable for Immunoblotting. In immunofluorescence and dot plot analysis, a week cross-reactivity was observed with AAV6 and AAV12. No reaction with other AAV types.
  The ADK1a antibody is used as capture and detection antibody in the AAV1 Titration ELISA (PRAAV1).
  
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  Background
  
  For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK1a specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.
  
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  AAV Antibodies
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG2a lambda
  
  Reactivity
  
  virus (AAV1, AAV6, AAV12)
  
  Clone
  
  ADK1a
  
  Immunogen
  
  AAV1 capsids
  
  Purification
  
  protein A affinity chromatography
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
  
  Storage
  
  short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
  
  * Research use only

Applications

Application

Affinity Chromatography; Dot Blots; ELISA; ICC/IF; IP; Neutralization Assay

Application	Dilutions
Affinity Chromatography	assay dependent
Dot Blot	assay dependent (non-denaturing conditions)
ELISA	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	1:20
Immunoprecipitation (IP)	1:5
Neutralization Assay	assay dependent

Application	Species	Publication
Affinity Chromatography	AAV1	PMID: 25410874
Dot Blots	AAV1 AAV6 AAV12	PMID: 24299301
ELISA	AAV1	PMID: 21917944
ICC/IF	AAV1 AAV6	PMID: 17126418
IP
Neutralization Assay

Reference
- Tseng, Y.-S. et al. Adeno-Associated Virus Serotype 1 (AAV1)-and AAV5-Antibody Complex Structures Reveal Evolutionary Commonalities in Parvovirus Antigenic Reactivity. J. Virol. 89, 1794-1808 (2015).
  Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).
  Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686-12697 (2011).
  Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17-24 (2007).

Datasheet

anti-AAV1 (intact particle) mouse monoclonal, ADK1a, lyophilized, purified

anti-AAV1 (intact particle) mouse monoclonal, ADK1a, lyophilized, purified

Background

More Details

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