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anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum

anti-AAV VP1/VP2/VP3 rabbit polyclonal (VP51), serum

Catalog Number:

A001003120PRO

Mfr. No.:

61084

Price:

$709

Size:

250 µL

Quantity:

Add to Cart:

Overview
- Reacts with VP1, VP2 and VP3. Raised against recombinant AAV2 capsid proteins.
  
  Please contact us at for specific academic pricing.
  
  Background
  
  The antibody reacts with VP1, VP2, and VP3 of adeno-associated virus (AAV).
  Rabbit polyclonal antibody (VP51).
  
  More Details
  
  AAV Antibodies
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Reactivity
  
  virus (AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10, AAV11, AAV12)
  
  Immunogen
  
  AAV2 capsids
  
  Purification
  
  stabilized antiserum
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  contains 0.09% sodium azide
  
  Storage
  
  short term at 2-8°C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
  
  Note
  
  centrifuge prior to opening
  
  * Research use only

Applications

Application

ELISA; ICC/IF; IP; WB

Application	Dilutions
ELISA	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	1:50 – 1:100
Immunoprecipitation (IP)	1:10
Western Blot (WB)	1:200 – 1:500

Application	Species	Publication
ELISA	AAV2	PMID: 28768875
ICC/IF	AAV1 AAV2 AAV3 AAV4 AAV5 AAV6 AAV7 AAV8 AAV9 AAVrh10 AAV11 AAV12	PMID: 24299301
IP	AAV2	PMID: 23015698
WB	AAV1 AAV2 AAV3 AAV4 AAV5 AAV6 AAV8 AAV9	PMID: 21917944

Reference
- Grosse, S. et al. Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells. J.Virol. 91,Â (2017).
  Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).
  Naumer, M. et al. Properties of the Adeno-Associated Virus Assembly-Activating Protein. J. Virol. 86, 13038-13048 (2012).
  Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686-12697 (2011).
  Kuck, D., Kern, A. & Kleinschmidt, J. A. Development of AAV serotype-specific ELISAs using novel monoclonal antibodies. J. Virol. Methods 140, 17-24 (2007).
  Kuck, D. et al. Intranasal Vaccination with Recombinant Adeno-Associated Virus Type 5 against Human Papillomavirus Type 16 L1. J. Virol. 80, 2621-2630 (2006).
  Grimm, D., Kay, M. A. & Kleinschmidt, J. A. Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6. Mol. Ther. 7, 839-850 (2003).

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