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Overview
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The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E. coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
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- Properties
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Overview
Immunofluorescence analysis of 293T cells using AARS antibody (STJ117211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse liver using AARS antibody (STJ117211) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using AARS antibody (STJ117211) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using AARS antibody (STJ117211) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using AARS Rabbit pAb (STJ117211) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
Anti-AARS antibody
Anti-AARS antibody
Catalog Number:
A001024058STJ
Mfr. No.:
STJ117211
Price:
$297
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