Aminoallyl-dUTP

- Overview
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    Background
    
    Aminoallyl-dUTP is used for indirect stable labeling of DNA/cDNA by polymerase chain reaction (PCR) with Taq polymerase, Nick Translation with DNAse I/ DNA Polymerase I, Primer Extension with Klenow exo-, 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) and Reverse Transcription with MMLV Reverse Transcriptase. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that introduces a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks or introduces fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging and dot blots (cDNA probes) [1][2].
    Aminoallyl-dUTP can also be introduced to probe by PCR and functions as a reactive site for subsequent labeling of the probe. The fluorescent labeling probe is used for in situ hybridization [2].
    
    [1] Dirsch et al. (2007) Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes. Appl. Immunohistochem. Mol. Morphol. 3:332.
    [2] Cox et al. (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. BioTechniques 36:114.
- Properties
  - Categories
    
    Labeling of DNA/cDNA via PCR and functions as a reactive site for subsequent labeling by reactive fluorescent probes.
    
    Alternative Name
    
    5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate,aa-dutp ,aa dutp,5-AA-dUTP,5-Aminoallyl-2'-deoxyuridine-5'-Triphosphate; ((2R,3S,5R)-5-(5-((E)-3-aminoprop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate
    
    CAS Number
    
    116840-18-7
    
    Molecular Formula
    
    C12H20N3O14P3(free acid)
    
    Molecular Weight
    
    523.22(free acid)
    
    Appearance
    
    Solution
    
    Purity
    
    98.00%
    
    Solubility
    
    Soluble in sterile water
    
    Storage
    
    Store at -20°C or below
    
    SMILES
    
    O=C(N1)N([[email protected]@H]2O[[email protected]](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)[[email protected]@H](O)C2)C=C(/C=C/CN)C1=O
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Protocol

Aminoallyl-dUTP

Background

Documents

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Aminoallyl-UTP