Aminoallyl-UTP

Aminoallyl-UTP

Catalog Number:
NNNA373426APE
Mfr. No.:
APE-B7951
Price:
$228
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      • Overview
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          Background

          Aminoallyl-UTP is used for Nucleic Acid Sequence Based Amplification (NASBA), Microarray based hybridization detection, Eberwine mRNA amplification and RNA-labeling. It is enzymatically incorporated into RNA during the NASBA reaction and Eberwine mRNA amplification. The resulting amine-functionalized RNA can subsequently be labeled via the classic Amine/NHS Ester reaction that introduces fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microarray based hybridization detection.

          [1] Scheler et al. (2009) Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications. BMC Biotechnol. 9:45.
          [2] Hoen et al. (2003) Fluorescent labelling of cRNA for microarray applications. Nucleic Acids Res. 31 (5):e20.

      • Properties
        • Categories
          Used for the production of labelled cDNA and cRNA for fluorescence detection in microarray.
          Alternative Name
          5-(3-Aminoallyl)-uridine-5'-triphosphate,aa-utp,aa utp,5-AA-UTP,5-Aminoallyluridine-5'-Triphosphate; ((2R,3S,4R,5R)-5-(5-((E)-3-aminoprop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate
          CAS Number
          112131-73-4
          Molecular Formula
          C12H17N3O15P3 (Anion)
          Molecular Weight
          536.19(Anion)
          Appearance
          Solution
          Purity
          98.00%
          Solubility
          Soluble in sterile water
          Storage
          Store at -20°C or below
          SMILES
          O=C(N1)N([[email protected]@H]2O[[email protected]](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)[[email protected]@H](O)[[email protected]]2O)C=C(/C=C/CN)C1=O

          * For Research Use Only

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