Amine Treated - 96 well plates - 8 Well Breakable Strips on single well holding frame

Amine Treated 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.

The Aminated Plates in various formats, are ideal for binding peptides and other molecules through covalent bonds. The surface of these Amine Treated 96-Well Plates is manufactured with primary amines, facilitating covalent immobilization of compounds containing reactive groups such as amino, carboxyl, or thiol groups, using well-established homo- and heterobifunctional linkers.

This covalent immobilization can overcome some of the limitations connected with physical adsorption of the molecules to the surfaces such us small peptides (M.W. 1,000-5,000 Da) drugs, toxins or hormones and can provide an oriented immobilization of molecules in order to secure the integrity and accessibility of their specific sites (e.g. Fab-SH-antibody fragments, streptavidin, or nucleic acids).

Aminated 96-Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The surface of Amine Treated 96-Well Plates with primary amino groups promotes covalent immobilization of compounds containing reactive moieties such as amino, carboxyl or thiol groups via well-known homo-heterobifunctional linkers.

The covalent immobilization can overcome some of limitations connected with physical adsorption of the molecules to the surface such as:

Small peptides (1,000-5,000 Da), drugs, toxins, or hormones which are bound weakly or not at allOriented immobilization of molecules in order to secure the integrity and accessibility of their specific sites avoiding the risk of inhibition of these sites by casual physical adsorption for such molecules as Fab-SH-antibody fragments, streptavidin, polysaccharides or nucleic acid (single strand or double strand) Enhanced storage stability by reducing the risk of desorption compared to physical adsorption methods.

Key features of Amine Treated Plates
· Amine Treated Surface: ideal for binding peptides and other molecules through covalent bonds.
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 75 to 200 μl
· Unit: 5 plates in a transparent bag
· Minimum order: 10 plates
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Amine Treated Surface promotes the covalent immobilization of compounds containing reactive moieties via well-known homoheterobifunctional linkers.
Surfaces with primary amino groups covalently bound are dedicated to promote the covalent immobilization of compounds containing reactive moieties such as amino, carboxyl or thiol groups via well-known homoheterobifunctional linkers, e.g. N-Hydroxysuccinimide (NHS) or Succinimidyl 4-(N-maleidomethyl) cyclohexane-1-carboxylate (SMCC).

This kind of immobilization can overcome some of the limitations connected with physical adsorption of the molecules to the surfaces such us:

immobilization of molecules which are bound weakly or not all by physical adsorption, namely small peptides (M.W. 1,000 – 5,000 Da) drugs, toxin or hormones
oriented immobilization of molecules in order to secure the integrity and accessibility of their specific sites avoiding the risk of inhibition of these sites by casual physical adsorption for such molecules as Fab-SH-antibody fragments, streptavidin, polysaccharides or nucleic acid (single strand or double strand)
increased storage stability compared with that of physical adsorption because of the reduced risk of spontaneous desorption
Introduction to the preparation and use of aminated surfaces for immunological assays
Several recipes are routinely used for the coupling of biological molecules to amino groups. Specific directions for use require the knowledge of the intended application. As a general guideline, the interaction between the amino group on the surface and the functional group of the molecule to be bound is based on covalent binding mediated by homo and heterofunctional crosslinkers.

In particular, Ethyldiethylaminopropylcarbodiimide (EDC), with or without the addition of Sulfo N-hydroxysuccinimide, is a powerful coupling agent of the carboxylic group of the molecule with the amino group of the surface.

If the biomolecule to be bound contains ε amino groups of lysine, the simplest method is coupling via Glutaraldehyde, with the formation of a stable amine linkage by reduction with Sodium Cyanoborohydride.

Other crosslinkers for this purpose are Dimethylpimelidate and Dissucinimidyl suberate.

Biomolecules containing thiolic groups, as Fab-SH or peptides with cystein at terminal end, can exploit the large number of maleimido groups containing crosslinkers as Succinimidyl 4-(N-maleimidomethyl)cycloexane-1-carboxylate (SMCC) for reacting with the amino group.

General directions for the use of surfaces with amino and carboxylic groups
The aminated surfaces offer the possibility to:

covalently immobilize small molecules which only bind weakly or not at all by physical adsorption
orientate the immobilization of molecules in a defined way on the solid phase
Hereunder are some examples of applications that can be used as guidelines to enable users to develop their own bio-specific assays.

· Amine Treated Surfaces main features
Biomat Aminated (primary amine) products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
Aminated Microplates Specifications
Treatment
The surface is modified with primary amino groups.
Volume of treatment: 200 μl/well.

Storage and Stability
The Aminated products are stored at room temperature in sealed PE bags and are stable until the expiration date printed on the label.