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Overview
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Aldol® 515 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding intense red color and red fluorescence. Aldol® 515 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 515 phosphate can be used under aerobic and anaerobic conditions.
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