Agrobacterium Competent Cells Preparation Kit

Agrobacterium Competent Cells Preparation Kit is specially developed for the rapid preparation of Agrobacterium competent cells. After the Agrobacterium is treated with this kit, the permeability of its cell membrane is temporarily changed, allowing the foreign DNA molecules to enter.

Agrobacterium often lives on the surface of plant roots and relies on the nutrients from roots to survive. It is a gram-negative bacteria ubiquitous in soil, and is often used to generate transgenic plants for overexpression, knockout, knockdown, or editing of target genes. After plant transformation, approximate 8 genes on the T-DNA of the Ti plasmid will be expressed in plant cells to guide the synthesis of a special compound opine which induces the tumorigenesis of transformed cells. The Ri plasmid of Agrobacterium Rhizogenes also contains T-DNA that can induce hairy roots which are characterized by a massively proliferating, highly branched root system.

This kit is applicable to most common Agrobacteria, such as Agrobacterium tumefaciens including GV3101, EHA101, LBA4404 and EHA105, or Agrobacterium rhizogenes such as C58C1 and Ar.1193. Competent cells prepared with this kit have a transformation efficiency of 103-104cfu/μg plasmid, which varies depending on the type of Agrobacteria. Moreover, the transformation efficiency for smaller plasmids is generally higher than for larger plasmids .

This kit is easy-to-use, requiring 1 hour approximately to obtain the competent cells after the bacterial culture is ready for preparation.

This kit provides a culture medium specially used to prepare Agrobacterium competent cells, which makes the preparation of competent cells more convenient.

Please refer to Figure for the performance of the Agrobacterium competent cells prepared by using this kit.

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