Name: Ag85A Recombinant Protein
Price: Inquiry

- Overview
  - Recombinant Mycobacterium tuberculosis Ag85A (43-338 a.a) produced in Hi-5 cells is a single, glycosylated polypeptide chain having a molecular mass of 32.8kDa (305 a.a in total). Antigen 85A is fused to a His tag at C-terminus and purified by conventional chromatographic techniques. Antigen 85A is a member of the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex have mycolyltransferase activity and catalyze the synthesis of the very rich glycolipid of the mycobacterial cell wall, the cord factor (trehalose 6,6-dimycolate, TDM). The cord factor is vital for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6-monomycolate (TMM) by Antigen 85A.
    
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- Properties
  - Protein Name
    
    Ronectin-binding protein A, Mycolyl transferase 85A.
    
    Source
    
    Baculovirus
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 90% as determined by SDS-PAGE.
    
    Formulation
    
    The Ag85A solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
    
    Storage
    
    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Ag85A Recombinant Protein

Ag85A Recombinant Protein

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