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Overview
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Fluorogenic substrate for caspase-3.
Ac-DEVD-AMC is a fluorogenic substrate for caspase-3 with a Km value of 9.7 1.0 μM. The sequence of Ac-DEVD-AMC is based on the PARP cleavage site Asp216 for caspase-3 and it is also a substrate for caspases -1, -4, -7 and -8. Ac-DEVD-AMC can be used to monitor intracellular caspase activity with cleaved AMC moieties, reflecting caspase activity, being detected by fluorescence.
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Overview