abberior LIVE 590, HaloX, 10 nmol

abberior LIVE 590 is a member of a new class of fluorescent dyes for live-cell imaging exclusively offered by abberior. This dye is highly photostable and bright orange fluorescent dye, which can be effectively excited with excitation light between 550 - 610 nm. For STED, a depletion wavelength between 750 - 800 nm is recommended. Due to the unique isomeric structure the favorited conformation is stabilized, leading to an enhanced cell permeability of the dye. abberior LIVE 590 can therefore be used in very low nanomolar concentration, reducing background and cytotoxicity effectively, allowing long-term STED and confocal imaging of living cells or tissue.

Our abberior LIVE 590 HaloX® probe can reversibly bind the active site of your HaloTag7 fusion protein in living cells and at the cell surface. Due to a high affinity, the probes interact with the HaloTag® protein but exchange regularly. This is made possible by preventing the formation of a covalent bond and allowing free exchange of fresh probes. In confocal and STED microscopy, this transient binding ensures that proteins can be detected in the living cell over longer periods of time, as bleaching processes are no longer crucial. This product contains 1 vial of abberior LIVE 590 HaloX as lyophilized powder, a stock solution of 1 mM in DMF or DMSO is recommended. Depending on cell type, tissue, and expression rate of the HaloTag7 fusion protein a working solution of 100 nM to 1 µM is advised.

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