AAV Capsid Barcode Kit-BBB

AAV Capsid Barcode Kit-BBB is designed to systematically evaluate different AAV serotypes or variants of their activity to cross blood-brain-barrier (BBB). Gene therapy targeting CNS diseases faces significant challenges primarily due to the BBB, which restricts the entry of therapeutic delivery vehicles. AAV9 has become a focal point in CNS gene therapy due to its ability to cross the BBB in mouse models. AAV9 serves as a foundational template for developing new AAV vectors with enhanced BBB penetration capabilities, such as AAV-Php.eB. However, AAV capsids that were modified for BBB crossing in mice have shown limited success in non-human primates (NHPs). Moreover, the species of mouse and NHP also affect the crossing-BBB activity of AAVs. This library includes a combination of 15 common AAV capsids and 9 reported crossing BBB AAV capsids, providing researchers with a comprehensive set of tools for targeted gene delivery to the brain. Researchers can conduct comparative studies to assess the transduction efficiency, specificity, and safety profiles of different AAV capsids targeting to CNS tissues using AAV Capsid Barcode Kit-BBB.

SKU:DH005001B
AAV Expression Cassette: AAV-CAG-EGFP-WPRE-BCs-bGHpolyA.
AAV Capsid List:

AAV status: Mixed viruses.
AAV Titer: ~1X10^13 VG/ml
BC design: (N)12-ACGGAAATACGATGTCGGGA-(N)12
Production: One AAV capsid and assigned 3 barcode plasmids were co-transfected to make one specific capsid AAV vector.
Purification: Each AAV capsid were produced(HEK 293T), purification(two rounds of CsCl) and titration(qPCR), separately.
Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
Storage: -80°C for long term (> 1 year); -20°C for short term (1-2 months); 4°C for 1-2 weeks.
Shipping: Dry ice.

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