AAV Capsid Barcode Kit-Common-CMV-mCherry

AAV Capsid Barcode Kit-Common-CMV-mCherry

Catalog Number:
PAP1554451AAV
Mfr. No.:
DA001006
Price:
$8,399
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      • Overview
        • AAV Capsid Barcode Kit-Common is designed to systematically evaluate different common used AAV serotypes or variants of their activities in different cells and tissues. This library includes 15 common AAV capsids. Researchers can use the unique DNA barcodes to evaluate the distribution of each capsid variant for different types of target cells.
          In the AAV Capsid Barcode Kit-Common-CMV-mCherry, the CMV-mCherry is used as reporter system. The CMV promoter is known for strong and ubiquitous expression in various cell types. The presence of the mCherry reporter gene allows for the enrichment of transgene-expressing cells, making it possible to identify and isolate cells that have taken up and expressed the AAV genomes. WPRE enhancer and bGH polyA can enhance mCherry expression and stabilize mRNA. In this kit, each AAV capsid is assigned to 3 different barcodes (BCs), which are located between WPRE and PolyA. Researchers can check both DNA and RNA levels with NGS targeting to BC region.

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      • Properties
        • Other Properties
          AAV Expression Cassette: AAV-CMV-mCherry
          AAV Capsids: AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, AAV2-Retro.
          AAV deliver packaging: Mixed viruses.
          AAV Titer: ~1X10^13 VG/ml.
          BC design: N25
          Production: One AAV capsid and assigned 3 barcode plasmids were co-transfected to make one specific capsid AAV vector using HEK 293T cells.
          Purification: Each AAV capsid were purified with two rounds of CsCl ultracentrifuge and titration with qPCR.
          Storage Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
          Storage
          -80°C for long term (> 1 year); -20°C for short term (1-2 months); 4°C for 1-2 weeks.
          Shipping
          Dry ice.
      • Applications
        • Application Description
          Tissue-specific AAV capsid Barcode Kits are designed to systematically evaluate AAV capsid variants that have been engineered to exhibit enhanced tropism for specific target tissues. It also includes 15 commonly used AAV serotypes that serve as controls, which are essential for benchmarking and comparative purposes.

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