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Overview
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For the quantitative enzymatic determination of uric acid in serum. Uric acid measurements are useful in the diagnosis of numerous renal and metabolic disorders. Uric acid is a substrate for the enzyme uricase. Uric acid is oxidized by oxygen in a reaction catalyzed by the enzyme uricase forming hydrogen peroxide and allantoin. Production of hydrogen peroxide can be assayed by 4-aminoantipyrine (4-AAP) in combination with 2-hydroxy-3,5-dichloro-benzenesulfonate (HDCBS) in the presence of horseradish peroxidase (HRP) through the production of an intensely red dye.
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![1,6,6-Trimethyl-6,7,8,9-tetrahydrophenanthro[1,2-b]furan-10,11-dione](https://resource.amerigoscientific.com/p-img/FT09489_structure.png)
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