6xHis-Ube2I

- Overview
  - Ube2I (Ubc9) is an E2 enzyme in the E1, E2, and E3 cascade to link SUMO to a substrate. This E2 shares a similar sequence and structure to the Ub E2s and is the only known SUMO conjugating enzyme at this time. Ube2I shares 56% amino acid identity with the yeast homolog called Ub – conjugating enzyme 9. While a hypothesis has been proposed that Ube2I may not need an E3 to ligate SUMO.
    
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- Properties
  - Protein Name
    
    P18; UBC9; C358B7.1
    
    Source
    
    Bacterial recombinant
    
    Formulation
    
    20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    See tube label
    
    Molecular Weight
    
    18 kDa
    
    Sequence
    
    NCBI RefSeq: NM_003345
    
    * Research Use Only