6xHis-Ube2F

- Overview
  - Ube2F is an E2 enzyme, which is part of the E1, E2, and E3 cascade responsible for conjugation of Nedd8 to protein substrates. Neddylation is initiated by activating Nedd8 by the Nedd8 activating enzyme APPBP1/UBA3. Cullin is a well-known substrate for Neddylation. When NEDD8 is covalently bonded to the CRL, it stimulates its E3 Ub ligase activity. A new approach to cancer therapy involves hindering the Nedd8 conjugation pathway through inhibition of the NAE1/UBA3 enzyme.
    
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- Properties
  - Protein Name
    
    NCE2
    
    Source
    
    Bacterial recombinant
    
    Formulation
    
    20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    See tube label
    
    Molecular Weight
    
    21.1 kDa
    
    Sequence
    
    NCBI RefSeq: NM_080678
    
    * Research Use Only