5-Color Multiple Fluorescent Staining Kit (mIHC)

This 5-Color Multiple Fluorescent Staining Kit (mIHC) is suitable for multiplex immunofluorescence staining of tissue samples, including paraffin sections, frozen sections, cell slides, cell smears, and organoids. It is particularly suitable for multiplex fluorescent immunolabeling using primary antibodies from the same source, but can also be used with antibodies from different sources. The primary principle of TSA is based on tyramide signal amplification (TSA), hereinafter referred to as TSA technology. TSA utilizes the peroxidase reaction of tyramide (wherein a fluorescently labeled tyramide forms a covalent binding site under the catalysis of HRP and H₂O₂). This generates a large number of enzymatic reactions, which then bind to surrounding protein residues (including tryptophan, histidine, and tyrosine residues), forming a large amount of fluorescent deposits at the antigen-antibody binding site, thereby amplifying the signal. This kit uses multiple fluorescent dyes to label tyramide, resulting in strong fluorescence and stable signals, making it suitable for repeated immunolabeling and multiplex fluorescent staining.

Advantages
· 7-Plex Signals from a Single Slide
7 spectrally resolved TSA fluorophores (480-750 nm) for any experimental panel
· Flexible Antibody Selection
Compatible with primary antibodies from rabbit and mouse
· Broad Applications
Works with FFPE sections, frozen sections, cells, and organoids
· All-in-One Components
Complete kit with tyramides, buffers, reagents, and mounting medium
· Gentle Antibody Elution
Special buffer removes antibodies while preserving antigens and tissue integrity

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