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Overview
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4-Nitrophenyl 4,6-ethylidene-α-D-maltoheptaoside is a substrate used in a reference measurement method for α-amylase activity in the diagnosis of acute pancreatitis, approved by the International Federation of Clinical Chemistry (IFCC). The substrate is hydrolysed by serum α-amylase into shorter 4-nitrophenylated oligosaccharides and their free counterparts. These reaction products are channeled into an indicator reaction with α-glucosidase and are degraded into glucose and the chromophore, 4-nitrophenol.
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