4-Dimethylaminoazobenzene-4-carboxylic Acid

- Overview
  - Dabcyl acid 4-dimethylaminobenzene-4’-carboxylic acid is a dark quencher chromophore that absorbs in the visible region and serves as an acceptor in FRET applications. It is widely used to measure protease activity by labelling a suitable peptide substrate with a fluorophore donor and a dabcyl dark quencher as an acceptor. The fluorophore-quencher energy transfer is related to distance (normally within 10nm). The peptide is cleaved by the protease action and the dabcyl quencher diffuses away from the fluorophore, and the fluorescence is measured. In RT-PCR, it is used at the non-fluorescent 3’ terminal of oligonucleotides as a quencher.
    
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- Properties
  - Categories
    
    Fine Chemicals
    
    Alternative Name
    
    Dabcyl acid
    
    CAS Number
    
    6268-49-1
    
    Molecular Formula
    
    C15H15N3O2
    
    Molecular Weight
    
    269.3 g/mol
    
    MDL Number
    
    MFCD00058988
    
    Note
    
    There is no hazardous surcharge associated with this product.
    There is no special packaging charge associated with this product.
    
    * For research use only. Not for human or veterinary use
- Reference
  - Chen., et al. (2005). Enzymatic activity characterization of SARS coronavirus 3C-likeprotease by fluorescence resonance energy transfer technique. Actapharmacologica Sinica 26: 99–106
    Kovaliov., et al. (2014). Synthesis and evaluation of a photoresponsive quencher forfluorescent hybridization probes. Organic & biomolecular chemistry 12:7844-7858
    Shang., et al. (2015). Biochemical Characterization of Recombinant Enterovirus 71 3C Protease with Fluorogenic Model Peptide Substrates and Development of a Biochemical Assay. Antimicrobial Agent Chemotherapy 59(4): 1827–1836.