3'-Amino Modifier TFA Amino C-6 lcaa CPG 500A

Amino Modifiers

This material is sensitive to heat and moisture. It requires the fastest shipment possible.

Amino Modifiers:

We offer wide variety 5'- or 3'-end or internal amino introducing amidites and supports. The standard DNA synthesis protocols are followed to quantitatively generate the primary aliphatic amino group. This primary aliphatic amino group is useful for the subsequent introduction of various functional entities such as Biotin, Fluorescent molecules, enzymes, amino acids, hydrophobic moieties, chelating agent etc. The primary aliphatic amino group reacts very smoothly with a wide variety of labeling reagents; thus it is the chemical modification of choice for this application. Thus cova­lently linked chromophores can be readily introduced in a synthetic DNA or RNA of interest.1 The 5’-Terminal amino Introducing Reagent such as C-4 Spacer (TFA-amino C-4, Catalog# CLP-1453), C-5 Spacer (Catalog# CLP-1357), C-6 Spacer (Catalog# CLP-1553) allows only for a single primary amino function per molecule. The amino group is introduced while protected as trifluoroacetamido (TFA) which is cleaved during ammonia deprot­ection of the oligo. The 5’-Terminal Amino Introducing Reagents allow for introduction of C-4, C-5 and C-6 spacer respectively between the 5’-phosphate and the terminally introduced primary amino group.

References:

1. Nelson, P. S.; Gold, R. S.; Leon, R. Nucl. Acids Res. 1989, 17, 7179