2',3'-Diacetyl Uridine CED phosphoramidite

2',3'-diacetyl 5'-Amidites

C22H33N4O9P

528.490

This material is sensitive to heat and moisture. It requires the fastest shipment possible.

Terminal Diol Generating Phosphoramidites:

2’,3’-O-Diacetyl-5’-phosphoramidites are available for the introduction of 2’,3’-ribose units at the 5’-end of oligonucleotides. Following ammonia deprotection, the 2’,3’-diacetate generates a free ribose 2’,3’-diol moiety in the oligonucleotide. Periodate cleavage of these diols generates dialdehydes.1 These dialdehydes have been stabilized by amines, especially by terminal amino groups of lysine residue of proteins or polypeptides.2 Such oligonucleotide-protein conjugates have been utilized in immuno assay and diagnostic applications.

Reference:

1. Krynetskaya, N. F.; Zayakina, G. V.; Oretskaya, T. S.; Volkov, E. M.; Shabarova, Z. A. Nucleosides Nucleotides Nucleicacids 1986, 5, 33.

2. Khym, J. X. Biochemistry 1963, 2, 344.