ZediXcite Fluorogenic TG2-Assay Kit

Assay Principle
TISSUE TRANSGLUTAMINASE (TG2) cleaves a dark quenching molecule from the side chain of TG2 specific peptide A163 incorporating glycine methyl ester. Subsequently, the fluorescence of an N-terminal coupled dye increases and can be monitored (excitation wavelength 330 nm; emission wavelength 418 nm).

Reagents in the kit
(1) SUBSTRATE REAGENT (SR): 2 x 23 μL TG2 specific peptide A163 (DMSO solution).
(2) BUFFER REAGENT (BR): 2 x 18 mL TRIS buffer pH 7.5 containing calcium chloride, sodium chloride, glycine methyl ester (lyophilizate).
(3) DILUTION BUFFER (DB): 1 x 18 mL TRIS buffer pH 8.1 containing sodium chloride, EDTA, DTT (lyophilizate).

Equipment
The ZediXcite Fluorogenic TG2-Assay Kit (F014) can be used in fluorescence microplate readers using microplates as well as in standard fluorescence spectrophotometers with cuvettes. Refer to the instructions of the manufacturer.

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