Name: Z-WEHD-FMK
Price: 292.00 USD

- Overview
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    Background
    
    Treatment of infected cells with pan-caspase inhibitor IV and Z-WEHD-FMK, an inhibitor of inflammatory caspases, elicited a near-complete blockage of C. trachomatis-induced cleavage of golgin-84. Golgin-84 cleavage was blocked via treatment of infected cells with Z-WEHD-FMK, resulting in a lack of Golgi fragmentation and a 2-log reduction in numbers of infectious bacteria.
    We treated infected cells with Z-WEHD-FMK, effectively preventing Golgi fragmentation, or with DMSO as a control and then labelled cells with fluorescent ceramide. Confocal images revealed that ceramide was rapidly incorporated into the inclusion membrane within DMSO-treated cells and accumulated inside the inclusion in bacterial membranes. In contrast, Z-WEHD-FMK-treated cells were only slightly fluorescent as the majority of lipid accumulated in a Golgi-like structure outside the inclusion1.
    General caspase inhibitor (Z-Asp-CHz-DCB) and capase-5 inhibitor (Z-WEHD-FMK) could not induce rRNA fragmentation treated with ECyd. Caspase-5 (ICErei Ill/TY), member of ICE protease, activated pathway may be concerned with ECyd induced rRNA fragmentation2.
    
    1. D. Heuer, A.R. Lipinski et al. Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction. NATURE, 457, 2009
    2. Kamada, S., Funahashi, Y. and Tsujimoto, Y.(1997) Cell Death Diff. 4, 473-478.
- Properties
  - Alternative Name
    
    Z-Trp-Glu(OMe)-His-Asp(OMe)-FMK; methyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-4-[[(2S)-3-(1H-indol-3-yl)-2-(phenylmethoxycarbonylamino)propanoyl]amino]-5-oxopentanoate
    
    CAS Number
    
    210345-00-9
    
    Molecular Formula
    
    C37H42FN7O10
    
    Molecular Weight
    
    763.77
    
    Purity
    
    98.00%
    
    Solubility
    
    insoluble in H2O; ≥26.32 mg/mL in EtOH with ultrasonic; ≥46.33 mg/mL in DMSO
    
    Storage
    
    Store at -20°C
    
    * For Research Use Only
- Reference
  - 1. Shouwen Chen, Peng Jin, et al. "Dual function of a turbot inflammatory caspase in mediating both canonical and non-canonical inflammasome activation." Dev Comp Immunol. 2021 Aug;121:104078. PMID:33794278
    2. WeiWei, Xiao-XueLi, et al. "Inhibition of vascular neointima hyperplasia by FGF21 associated with FGFR1/Syk/NLRP3 inflammasome pathway in diabetic mice." Atherosclerosis. Available online 29 August 2019.
    3. Yang D, Zheng X, et al. "Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish." Nat Commun. 2018 Aug 3;9(1):3052. PMID:30076291

Z-WEHD-FMK

Z-WEHD-FMK

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