YLEX Yeast Expression Kit

YLEX Yeast Expression Kit

Catalog Number:
BE01547404YEA
Mfr. No.:
FYY201-1KT
Price:
  • Size:
    1 kit
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      • Overview
        • YLEX Expression Kit based on INRA INAPG licensed patent* provides an easy approach for cloning and expressing a gene of interest in the yeast, Yarrowia lipolytica. Using this kit, high level of heterologous protein may be expressed intracellularly or be secreted from the cell into the medium by selecting the supplied expression vector pYLEX1 or pYLSC1.
          * INRA (Institut National de la Recherche Agronomique) and INAPG (Institut National Agronomique Paris-Grignon)

          Features
          • Safe: Y. lipolytica was classified as GRAS (Generally Regarded As Safe) by the US FDA (Food and Drug Administration)
          • Simple: a simple tool for expressing heterologous protein
          • Easy manipulation: like E. coli and S. cerevisiae
          • Stable: strong stability in vectors and constructed plasmids
          • Reliable: vectors integrated at the same site in genome
          • Flexible: both expression and secretion vector provided (proteins may be expressed intracellularly or be secreted from the cell into medium)
          • High growth ability: high secretion capacity & high product yield
          • Less protein degraded: no extracellular protease synthesized by a special protease-deficient Yarrowia strain
          • Mass production: industrial mass production of recombinant proteins
          • Less hyperglycosylation: able to perform post-translational processing of complex proteins, unlike S. cerevisiae

          Please contact us at for specific academic pricing.

      • Properties
        • Other Properties
          Genotype: MatA, leu2-270, ura3-302::URA3, xpr2-332, axp-2
          Phenotype: Leu-, ΔAEP, ΔAXP, Suc+, pBR platform
          Storage
          4 °C

          * For Research Use Only.

      • Applications
        • Application Description
          Heterologous protein expression, either intracellular or extracellular depends on selected vector in GRAS yeast.
          Yarrowia Vectors
          Two vectors (pYLEX1 and pYLSC1) are included in this kit, and they can be used for either intracellular expression or secretion of proteins of interest in Y. lipolytica. Generally speaking, if the target protein is cytosolic and non-glycosylated, the pYLEX1 vector is a better choice. If the protein of your interest is normally glycosylated or secreted, you may want to choose the pYLSC1 vector.

    Documents

    Manual

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