Wnt Signaling Phospho Antibody Array

Wnt Signaling Phospho Antibody Array features 227 highly specific antibodies important to the Wnt/β-Catenin pathway. The array is designed for protein phosphorylation profiling in cell and tissue samples in human, mouse, or rat. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

Wnt signaling has a direct association with cancer, type II diabetes, and neurodiseases. Signaling of the highly secreted glycolipoproteins in the Wnt family controls embryonic development and adult homeostasis by regulating β-Catenin's accumulation in the cytoplasm. When Wnt signaling is absent, β-Catenin is targeted for ubiquitination and degraded by the destruction complex formed by Axin, APC, GSK3, PP2P, and CK-1α. As a result, β-Catenin cannot accumulate in the cytoplasm. When Wnt binds to Frizzled receptors and LRP5/6, Wnt signaling is turned on and disrupts the destruction complex's function. Now β-Catenin accumulates, moves into the nucleus, and binds to TCF/LEF transcriptor factors to activate gene transcription.

Wnt Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine and serine/threonine phosphorylation at specific sites.

Key Features:
· Site-specific phosphorylation profiling and screening with 227 antibodies
· Antibodies covalently immobilized on 3D polymer coated glass slide
· Fluorescent detection

Specifications:
· Product Size: 2 array slides per package for two samples (untreated vs. treated)
· Featured Antibodies: 227 site-specific and phospho-specific antibodies; 6 replicates per antibody
· Reactivity: Human: 100% | Mouse: 92% | Rat: 80%
· Suitable Sample Type: Cell lysate | Tissue lysate
· Detection Method: Fluorescence | Compatible Scanners
· Internal Controls: Positive controls: beta-actin | GAPDH | Negative controls
· Slide Dimensions: 76 x 25 x 1 mm

Target Proteins:
AKT (Ser124), AKT (Ser246), AKT (Ser473), AKT (Thr308), AKT (Thr450), AKT (Thr72), AKT (Tyr326), AKT (Tyr474), AKT1 S1 (Thr246), AKT1/2/3 (Tyr315/316/312), AKT2 (Ser474), APC (Ser2054), APC1 (Ser355), APC1 (Ser688), CaMK2a (Thr286), CaMK2a/b/d (Thr305), CaMK2b/g/d (Thr287), Catenin a1 (Ser641), Catenin beta (Ser33), Catenin beta (Ser37), Catenin beta (Thr41/Ser45), Catenin beta (Tyr489), Catenin beta (Tyr654), Catenin delta1 (Tyr228), CKI-a (Tyr294), CKI-a (Tyr321), CKI-g 1/2/3 (Tyr263), CKII-a (Tyr255), CKII-b (Ser209), ERK3 (Ser189), ERK8 (Thr175/Tyr177), GPR120, GPR132, GPR150, GPR151, GPR152, GPR153, GPR160, GPR171, GPR173, GPR174, GPR175, GPR18, GSK3a (Ser21), GSK3a/b (Tyr216/279), GSK3b (Ser9), JNK1/2/3 (Thr183/Tyr185), MAP3K1 (Thr1402), MAP3K10, MAP3K11 (Ser674), MAP3K11 (Thr277/Ser281), MAP3K15, MAP3K3, MAP3K4, MAP3K6, MAP3K7 (Ser439), MAP3K71 (Thr187), MAP3K8 (Ser400), MAP3K9, MAP3KL4, MEK1 (Ser217), MEK1 (Ser221), MEK1 (Ser298), MEK1 (Thr286), MEK1 (Thr291), MEK2 (Thr394), MEKKK1, MEKKK4, mTOR (Ser2448), mTOR (Ser2481), mTOR (Thr2446), NFAT1 (Ser326), NFAT3 (Ser168/Ser170), NFAT3 (Ser676), NFAT4 (Ser165), NFAT5 (Ser1197), P38 MAPK (Thr179/Tyr181), P38 MAPK (Thr180), P38 MAPK (Tyr182), P38 MAPK (Tyr322), P44/42 MAPK, PDE4D (Ser190/53), PI3K-p85-a (Tyr607), PI3K-p85-a/g (Tyr467/Tyr199), PKA-a/b CAT (Thr197), PKA-R2-b (Ser113), PKC-a (Thr638), PKC-a (Tyr657), PKC-b (Ser661), PKC delta (Ser645), PKC delta (Thr505), PKC delta (Tyr313), PKC delta (Tyr52), PKC delta (Tyr64), PKC epsilon (Ser729), PKC pan (Thr497), PKC theta (Ser676), PKC theta (Ser695), PKC theta (Thr538), PKC zeta (Thr410), PKC zeta (Thr560), PKG2 (Ser126), PLC beta 3 (Ser1105), PLC beta 3 (Ser537), PLCG1 (Tyr1253), PLCG1 (Tyr771), PLCG1 (Tyr783), PLCG2 (Tyr1217), PLCG2 (Tyr753), PLCG2 (Tyr759), Rac1/cdc42 (Ser71), Rho/Rac GEF2 (Ser885), RhoA (Ser188), SAPK/JNK (Thr183), SAPK/JNK (Tyr185), Src (Ser75), Src (Tyr216), Src (Tyr418), Src (Tyr529), TAK1 (Thr184), Ubiquitin, VE-Cadherin (Tyr731), WNT1, WNT5A

Assay Outline:
The ELISA based Wnt Signaling Phospho Antibody Array platform involves four major steps:
· Protein extraction with non-denaturing lysis buffer
· Biotinylation of protein samples
· Incubation of labeled samples with antibody array
· Detection by dye conjugated streptavidin

Please contact us at for specific academic pricing.

Background

· High-Throughput Antibody Arrays
Our antibody arrays provide a high-throughput, ELISA-based solution for protein expression profiling across samples like cells, tissues, serum, and culture media. It offers greater productivity and cost savings compared to traditional Western Blots or ELISA.

· Comprehensive Protein Profiling
Choose from our comprehensive arrays: Phospho Explorer Array, Signaling Explorer Array, and Explorer Antibody Array. Profile up to 1,358 biomarkers on a single slide, with options tailored to specific pathways in immunology, oncology, and cell biology.

· Phosphorylation Profiling
Our phosphorylation profiling antibody arrays include the Phospho Explorer Array with 1318 antibodies and Pathway Phospho Arrays covering more than 25 different pathways. This platform features phospho-specific antibodies and paired site-specific antibodies for total protein. It is ideal for tyrosine, serine, and threonine phosphorylation analysis.

· High-Quality, Reliable Arrays
Our antibody arrays feature antibodies covalently attached to premium glass with a 3D polymer coating for optimal binding and specificity. Each includes positive/negative controls and multiple replicates for accurate, reliable results.