WizPure™ Pfu DNA Polymerase

WizPure™ Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5'→3' direction.
Pfu DNA Polymerase also possesses 3'→5'exonuclease (proofreading) activity. WizPure™ Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, is even up to tenfold more accurate than normal Taq DNA polymerase. Consequently, WizPure™ Pfu DNA Polymerase is useful for polymerization reactions requiring high-fidelity synthesis.

Overview
• Error rate : 1.3 x 10-6
• Time to 1kb (72°C) : 60-120
• Thermal stability : Half-life of 4 hrs at 95°C
• Blunt end products
• Recombinant proteins expressed in E. coli
• Free of detectable non-specific nuclease

Features
250 U contains:
• 1 x 100 µl, Pfu Polymerase (2.5U/µl)
• 1 x 1 ml, 10X PCR Buffer
• [ 1 x 0.5 ml, dNTP mix (2.5 mM each) ]

500 U contains:
• 1 x 200 µl, Pfu Polymerase (2.5U/µl)
• 2 x 1 ml, 10X PCR Buffer
• [ 1 x 1 ml, dNTP mix (2.5 mM each) ]

2,500 U contains:
• 1 x 1,000 µl, Pfu Polymerase (2.5U/µl)
• 8 x 1 ml, 10X PCR Buffer
• [ 5 x 1 ml, dNTP mix (2.5 mM each) ]

Please contact us at for specific academic pricing.