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Overview
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WizPure™ HS-PCR FDmix combines all the reagents necessary for successful antibody-based hot-start PCR in a convenient individually aliquoted and lyophilized in single-tube. WizPure™ HS-PCR FDmix is an optimized, economical, highly efficient, and ready-to-use hot-start PCR premix that can amplify templates of up to 5 kb. The PCR mixture of hot-start Taq DNA Polymerase, PCR buffer, MgCl2, and dNTPs. This HS-PCR FDmix contains all components for PCR, except DNA template and primers. The mixture is suitable for amplification of most of the DNA templates. Tested for the absence of endonucleases & exonucleases and is also tested for amplification of single gene copy.
WizPure™ HS-PCR FDmix yields excellent and consistent results in routine PCR reactions as well as high-throughput PCR genotyping, colony PCR, RT-PCR, and PCR cloning. PCR reactions can be directly loaded onto an agarose gel without the additional need of loading buffer and dyes.
Overview
• Including loading dye for easy gel electrophoresis
• A-tail formation at 3’ ends of amplified duplex DNA
• Ready-to-use dried type
• Easy reaction setup (pre-aliquot in 8-strip tube)
• High PCR specificity and high sensitivity PCR amplification
• Antibody-mediated hot-start PCR
• Room-temperature reaction set-up
• Contains loading dye for visual confirmation and quick electrophoresis
• Minimal optimization requirements
• Eliminating extra pipetting steps and multiple reagents reduces contamination risk and provide better reproducibilityPlease contact us at for specific academic pricing.
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Overview