Vivo-Morpholinos - Modification with 5'-Fluor

A Vivo-Morpholino is a Morpholino with a covalently attached delivery moiety. The in vivo delivery moiety is an octoguanidine dendrimer which conjugates to the 3' end of a Morpholino oligo to enhance cell penetration. We offer further modification to the 5' end of Vivo-Morpholinos.

Please contact us at for specific academic pricing.

Background

Vivo-Morpholinos (US Patent 7,935,816) are the knockdown, exon-skipping or miRNA blocking reagent of choice for in vivo experiments. Outstanding results can be achieved systemically with intravenous (I.V.) injection, and modest systemic delivery achieved with Vivo-Morpholinos by intraperitoneal (I.P.) injection. Efficient localized delivery can be achieved by injecting the Vivo-Morpholino directly into the area of interest. Vivo-Morpholinos are also effective and easy to use in cultures.

Vivo-Morpholinos get the job done by combining two important parts

A Vivo-Morpholino is comprised of a Morpholino oligo with a unique covalently linked delivery moiety, which is comprised of an octa-guanidine dendrimer. It uses the active component of arginine rich delivery peptides (the guanidinium group) with improved stability and reduced cost. The Vivo-Morpholino is assembled by coupling the vivo-delivery group to a Morpholino while the oligo is still bound to its synthesis resin, allowing excellent purification by washing the solid-phase resin. Vivo-Morpholinos must be chosen prior to synthesis and cannot be added later because the vivo-delivery group is added to a Morpholino prior to cleavage from its synthesis resin.

All Vivo-Morpholinos are provided in 400, 2000, and 10000 nmole quantities in lyophilized form. We suggest you make a 0.5 mM solution in phosphate buffered saline; while solubility varies and is sequence-dependent, higher concentrations risk exceeding the solubility of the Vivo-Morpholino. All Vivo-Morpholinos are sterilized. The amount of oligo you will need depends on the experiment and the method of injection. For a typical 20g mouse injected at 12.5 mg/kg I.V. the dose is approximately 25 nmole per injection. This means you will get approximately 16 injections from the 400 nanomole quantity, 80 injections from the 2000 nanomole quantity and 400 injections from the 10000 nanomole quantity.