VB UltraStable™ Chemically Competent Cells

VB UltraStable™ Chemically Competent Cells

Catalog Number:
DC01456961VEC
Mfr. No.:
UC001-010
Price:
$448
  • Size:
    10x100 ul
    Quantity:
    Add to Cart:
      • Overview
        • VB UltraStable™ chemically competent cells are designed to achieve high transformation efficiency (>1x10⁹cfu/ug) and to propagate DNA plasmids with unstable elements such as repeated sequences. Whether you are doing routine cloning or high-throughput cloning, our host cells offer you the comprehensive technical solutions to meet a wide range of cloning needs.

          Highlights
          ▪ Lacks the ability to undergo homologous recombination due to the mutations introduced in the recA gene.
          ▪ Suitable for cloning and hosting of lentiviral, retroviral vectors and vectors with repeated sequences and unstable fragments.
          ▪ High efficiency for Gateway Cloning, because the cells lack the functional ccdAB operon.
          ▪ Can produce high quality plasmid DNA due to the endA mutation (the plasmids won't be digested by endonuclease).
          ▪ T1 phage resistant due to the fhuA mutation.
          ▪ Can be used for blue/white screening because it expresses the omega-fragment of the LacZ gene.

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Chemically Competent Cells
          Other Properties
          Genotype: F’[∆ccdAB proAB+ lacIq lacZ∆M15 zzf::Tn10 (TetR)] recA1 endA1 fhuA mcrA ∆(mrr-hsdRMS-mcrBC) Φ80dlacZ∆M15 ∆(ara, leu)7697 araD139 ∆lacX74 galK16 galE15 e14- relA1 nupG rpsL (StrR) rph spoT1
          Antibiotic resistance: Streptomycin, tetracycline
          Strain origin: E.coli K-12
          Storage
          Cryopreserved cells are shipped on dry ice. Upon receiving, competent cells should be stored at -80°C for long-term storage. S.O.C. medium and pUC19 plasmid DNA can be stored at -80°C for long-term storage, or at 4°C for up to two weeks.

          * For research use only.

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