UCF.ME™ UltraNuclease GMP-grade (250 U/μL)

UltraNuclease is a genetically engineered endonuclease from Serratia marcescens. The enzyme digests all forms of DNA/RNA moleculars into oligonucleotides of about 5 bp. The product is expressed in Escherichia coli (E. coli) and purified under the GMP environments. The nuclease is equivlent to benzonase in reducing host nucleic acid residues to pg-grade, improving the performance and safety of biological products of applications including virus purification, vaccine manufacturing, and protein/polysaccharide pharmaceutical manufacturing. It can also used in protein study to reduce the viscosity of cell supernatant and cell lysate, increase protein purification efficiency and enhance protein functional research.

UltraNuclease is provided in the form of a sterilized reagent, eluted in buffer (20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerin), with the appearance of a colorless, transparent liquid. This product is produced by GMP process requirements and provided in a liquid form.

Features
• Wide range of applications: degrade all forms of DNA and RNA
• High purity and activity: purity ≥ 99%; specific activity ≥ 1.5×106 U/mg
• Strong adaptability: strong tolerance, adapt to a variety of reaction conditions
• Comply with pharmacopeia: no animal origin; no antibiotics; lower endotoxin
• Qualification: product gets the FDA DMF filling which can shorten the customers for new drug application
• GMP-grade manufacturing: strict standards to meet the needs of large-scale use for research to manufacture

Unit Definition: the definition of one Activity unit (U) is the amount of enzyme used to change the absorption value of △A260 by 1.0 in 30 minutes in a 2.625 mL reaction system at 37°C with a pH of 8.0 (equivalent to complete digestion of 37 μg salmon sperm DNA into oligonucleotides).

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